Philippine Council for Agriculture, Aquatic and Natural Resources Research Development
Andali rapid diagnostic test kits are rapid, timely, and inexpensive kits dedicated to detect presence of African swine fever (ASF) virus, Classical Swine Fever (CSF) virus, Porcine Reproductive and Respiratory Syndrome (PRRS) virus, and Porcine Epidemic Diarrhea (PED) virus.
First is the ASFv Nanogold Biosensor Test Kit, which uses a combination of loop mediated isothermal amplification (LAMP) primers targeting the P72 gene responsible for viral attachment of the ASF virus and gold nanoparticles to stain the amplified products. It can be used to detect ASF from surface swabs of inanimate objects in farms and delivery trucks, water supplies in farms, infected bodily fluids (blood and saliva/oropharyngeal) and feces, as well as raw, processed, or canned pork products. The ASFV Nanogold Biosensor is completely packaged in a built-in DNA extraction method with corresponding extraction reagents included together with the Nanogold Premix and gold dye for the amplification reaction.
Second is the dry format of the reverse transcription-loop mediated isothermal amplification (RT-LAMP) test kit developed for CSF virus (or hog cholera) and PRRS virus detection. It is a novel modification of the original wet format as it eliminates the use of cold storage (dry ice or freezer) which you can bring anywhere, anytime without worries of reagent degradation, hence making it more convenient. The dry RT-LAMP is able to differentiate the infected from the vaccinated animal or DIVA. Hence, the RT-LAMP with DIVA features a better diagnostic test than RT-PCR which cannot differentiate the live attenuated vaccine viruses incorporated in commercial vaccines from the infective field strain. The test kit is a complete package which carries a built-in RNA extraction procedure with corresponding reagents and the RT-LAMP premixes with dye. After extracting the RNA from the sample, the RT-LAMP reaction can be completed after 30 mins at 600C.